Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO2Nanoparticles as Labels
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منابع مشابه
Electrochemical Immunoassay of Escherichia coli O157:H7 Using Ag@SiO2 Nanoparticles as Labels
Silica coated silver (Ag@SiO2) nanoparticles were prepared and characterized by transmission electron microscope (TEM) and UV-vis absorption, and the nanoparticles were used as labels in sandwich-type immunosensor of Escherichia coli O157:H7 (E. coli O157:H7). The labels involved in immunoreaction were dissolved by mixed acid of hydrofluoric acid and nitric acid, and the released Ag(+) ions wer...
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EXPERIMENTAL SECTION Materials and reagents Tetrabutyl titanate (TBOT), sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS) and phosphoric acid (H3PO4) were from Nanjing Chemical Reagent Co., Ltd. (Nanjing, China). Human IgG (HIgG) (Ag), rabbit anti-human IgG (Ab1), and goat anti-human IgG (Ab2) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China)....
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A novel horseradish peroxidase- (HPR-) doped magnetic core-shell Fe3O4@SiO2@Au nanocomposites (Fe-Au MNPs) were employed on immunoassay for the determination of C-reactive protein (CRP) based on a electrochemical quartz crystal microbalance detector (EQCM). Firstly, the secondary CRP antibody and HRP were both immobilized on the Fe-Au MNPs (Fe-Au MNPs-anti-CRP2/HRP) as a signal tag. Secondly, t...
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ژورنال
عنوان ژورنال: Journal of Analytical Methods in Chemistry
سال: 2014
ISSN: 2090-8865,2090-8873
DOI: 10.1155/2014/247034